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Protein Purification
Theory and Techniques
₹625.50 ₹695.00 Save: ₹69.50 (10%)
Go to cartISBN: 9789386243980
Bind: Paperback
Year: 2018
Pages: 232
Size: 8.5 x 11 Inch
Publisher: Viva Books Originals
Sales Territory: Worldwide
Description:
Protein Purification - Theory and Techniques provides a good reference for the techniques and strategies involved in the purification of antibodies and recombinant proteins. The detailed practical guidelines given in the book aim particularly at laboratory scale purification. The book is designed to give the laboratory worker the information needed to undertake the challenge of designing a strategy to produce a purified protein from a crude mixture or cell debris.
The book also reflects personal preferences amongst the multitude of methods that may be used for protein fractionation. There is no single correct way to solve the problem, and probably teams could choose several alternative procedures and come up with a satisfactory solution. The approach and the results are discussed and compared in this book.
Target Audience:
Undergraduates and Postgraduates of Biochemistry, Molecular Biology, Immunology, Genetic Engineering, Biotechnology and Microbiology.
Contents:
Preface
PART 1: PROTEIN PURIFICATION
Chapter 1: Protein Purification
Chapter 2: Preparation
Chapter 3: Three Phase Purification Strategy
Chapter 4: Capture
Chapter 5: Intermediate Purification
Chapter 6: Polishing
Chapter 7: Examples of Protein Purification Strategies
Chapter 8: Sample Storage Conditions
Chapter 9: Principles and Standard Conditions for Purification Techniques
PART II: ANTIBODY PURIFICATION
Chapter 10: Antibody Purification
Chapter 11: Sample Preparation
Chapter 12: Simple, Rapid Purification by Affinity Chromatography
Chapter 13: Immunoprecipitation
Chapter 14: Multi-Step Purification Strategies
Chapter 15: Removal of Specific Contaminants After Initial Purification
Chapter 16: Large-Scale Purification
PART III: RECOMBINANT PROTEINS
Chapter 17: Purification of Recombinant Proteins
Chapter 18: GST Fusion Proteins
Chapter 19: (His)6 Fusion Proteins
Chapter 20: Handling Inclusion Bodies
Chapter 21: Harvesting and Extraction of Recombinant Proteins
Chapter 22: Buffer Exchange and Desalting of Recombinant Proteins
Chapter 23: Simple Purification of other Recombinant Proteins
Chapter 24: Multi-Step Purification of Recombinant Proteins
APPENDIX 1
APPENDIX 2
APPENDIX 3
Index
About the Authors:
K. S. Dayananda is the Head of the Department of Post Graduate Studies in Biochemistry at Padmashree Institute of Information Sciences, Bangalore. He has been teaching biochemistry, molecular biology, immunology and genetic engineering to undergraduates and postgraduates for several years.
